Protozoal Digestion of Coat-Defective Bacillus subtilis Spores Produces “Rinds” Composed of Insoluble Coat Protein
نویسندگان
چکیده
منابع مشابه
Bacillus subtilis spore coat.
In response to starvation, bacilli and clostridia undergo a specialized program of development that results in the production of a highly resistant dormant cell type known as the spore. A proteinacious shell, called the coat, encases the spore and plays a major role in spore survival. The coat is composed of over 25 polypeptide species, organized into several morphologically distinct layers. Th...
متن کاملThe Influence of Sporulation Conditions on the Spore Coat Protein Composition of Bacillus subtilis Spores
Spores are of high interest to the food and health sectors because of their extreme resistance to harsh conditions, especially against heat. Earlier research has shown that spores prepared on solid agar plates have a higher heat resistance than those prepared under a liquid medium condition. It has also been shown that the more mature a spore is, the higher is its heat resistance most likely me...
متن کاملGel-free proteomic identification of the Bacillus subtilis insoluble spore coat protein fraction.
Species from the genus Bacillus have the ability to form endospores, dormant cellular forms that are able to survive heat and acid preservation techniques commonly used in the food industry. Resistance characteristics of spores towards various environmental stresses are in part attributed to their coat proteins. Previously, 70 proteins have been assigned to the spore coat of Bacillus subtilis u...
متن کاملStructural and germination defects of Bacillus subtilis spores with altered contents of a spore coat protein.
The start sites for transcription and translation of a Bacillus subtilis spore coat protein gene, cotT, were determined. The CotT protein was synthesized as a 10.1-kDa precursor which was processed to a coat polypeptide of 7.8 kDa. Insertional inactivation of the cotT gene resulted in spores with an altered appearance of the inner coat layers and slow germination in response to a germination so...
متن کاملAlternative translation initiation produces a short form of a spore coat protein in Bacillus subtilis.
During endospore formation in Bacillus subtilis, over two dozen polypeptides are localized to the developing spore and coordinately assembled into a thick multilayered structure called the spore coat. Assembly of the coat is initiated by the expression of morphogenetic proteins SpoIVA, CotE, and SpoVID. These morphogenetic proteins appear to guide the assembly of other proteins into the spore c...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2008
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01228-08